Reference Gene U2 Enables Direct Comparison between Relative Gene Expression Levels of Vascular Smooth Muscle Cells in Tissue and Culture Using Real-Time Quantitative PCR
In nearly every lab, real-time quantitative polymerase chain reaction (qPCR) is used to quantify gene expression.However, a comparison of different samples requires the careful selection of suitable reference genes (RGs), sometimes referred to as housekeeping genes.In the case of vascular smooth muscle cells (vSMCs), it is important to know under w